| IED ID | IndEnz0009000345 |
| Enzyme Type ID | chitinase000345 |
| Protein Name |
Cortical fragment-lytic enzyme CFLE EC 3.2.1.- Germination-specific spore peptidoglycan hydrolase Spore peptidoglycan N-acetylglucosaminidase |
| Gene Name | sleL |
| Organism | Bacillus cereus |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus cereus group Bacillus cereus |
| Enzyme Sequence | MIQIVTVRSGDSVYSLASKYGSTPDEIVKDNGLNPAETLVVGQALIVNTKGNNYYVQPGDSLYRISQTYNVPLASLAKVNNLSLKSILHVGQQLYVPKGTKRAVESIAYLQPSTIPIKESLVNATRAINPFLTYLAYFSFEAKRDGTLKEPTETAKIANIATQGKTIPMLVITNIENGNFSADLTSVILRDATIQNKFITNILQTAQKYGMRDIHFDFESVAPEDREAYNRFLRNVKTRLPSGYTLSTTLVPKTSSNQKGKFFEAHDYKAQGQIVDFVVNMTYDWGWQGGPPMAISPIGPVKEVLQYAKSQMPPQKIMMGQNLYGFDWKLPFKQGNPPAKAISSVAAVALARKYNVPIRYDFTAQAPHFNYFDENGVQHEVWFEDSRSVQSKFNLMKEQGIGGISYWKIGLPFPQNWRLLVENFTITKKG |
| Enzyme Length | 430 |
| Uniprot Accession Number | Q9K3E4 |
| Absorption | |
| Active Site | ACT_SITE 219; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01258 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by diethylpyrocarbonate. {ECO:0000269|PubMed:10692353}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.2.1.- |
| Enzyme Function | FUNCTION: N-acetylglucosaminidase involved in cortex peptidoglycan degradation during germination. Cleaves only partially degraded spore peptidoglycans. Recognizes muramic acid delta-lactam residues specific to spore peptidoglycans. {ECO:0000269|PubMed:10692353}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 6.0. {ECO:0000269|PubMed:10692353}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Domain (3) |
| Keywords | Direct protein sequencing;Germination;Glycosidase;Hydrolase;Repeat |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Spore cortex {ECO:0000269|PubMed:10692353}. Note=Probably localizes to the exterior of the cortex layer. {ECO:0000269|PubMed:10692353}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 48,138 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |