| IED ID | IndEnz0009000317 |
| Enzyme Type ID | chitinase000317 |
| Protein Name |
Soybean toxin 27 kDa chain SBTX 27 kDa chain Fragment |
| Gene Name | |
| Organism | Glycine max (Soybean) (Glycine hispida) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids fabids Fabales Fabaceae Papilionoideae 50 kb inversion clade NPAAA clade indigoferoid/millettioid clade Phaseoleae Glycine Glycine subgen. Soja Glycine max (Soybean) (Glycine hispida) |
| Enzyme Sequence | ADPTFGFTPLGLSEKANLQIMKAYD |
| Enzyme Length | 25 |
| Uniprot Accession Number | P86520 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Involved in plant defense (PubMed:18328522). Inhibits spore germination in C.sojina, A.niger (at concentrations >50 ug/ml) and P.herguei but not in F.oxysporum and F.solani (PubMed:20831249, PubMed:18328522). Does not inhibit vegetative mycelial growth (PubMed:20831249). Inhibits growth of C.albicans and K.marxiannus but not P.membranifaciens or C.parapsilosis (PubMed:20831249, PubMed:23894655). Probably acts by affecting the cell membrane (PubMed:20831249). Does not have urease, chitinase, beta-1,3-glucanase or hemagglutination activities (PubMed:18328522, PubMed:20831249). Does not inhibit trypsin (PubMed:18328522). Injection into mice produces toxic effects such as dyspnea, tonic-clonic convulsion and death (PubMed:18328522). {ECO:0000269|PubMed:18328522, ECO:0000269|PubMed:20831249, ECO:0000269|PubMed:23894655}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Activity decreases below pH 5.5 and above pH 8.0. {ECO:0000269|PubMed:18328522}; |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Non-terminal residue (1) |
| Keywords | Antimicrobial;Direct protein sequencing;Disulfide bond;Fungicide;Plant defense;Reference proteome;Toxin |
| Interact With | |
| Induction | INDUCTION: In seeds, induced by jasmonate. {ECO:0000269|Ref.2}. |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | PTM: SBTX is known to be glycosylated but it is not known which one of its two subunits is modified; contains 5% carbohydrates. {ECO:0000269|PubMed:18328522}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 2,728 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |