| IED ID | IndEnz0002019034 |
| Enzyme Type ID | protease019034 |
| Protein Name |
Serine protease EspP EC 3.4.21.- Cleaved into: Secreted autotransporter protein EspP Extracellular serine protease plasmid-encoded EspP ; Autotransporter protein EspP translocator |
| Gene Name | espP pssA |
| Organism | Escherichia coli |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli |
| Enzyme Sequence | MNKIYSLKYSHITGGLIAVSELSGRVSSRATGKKKHKRILALCFLGLLQSSYSFASQMDISNFYIRDYMDFAQNKGIFQAGATNIEIVKKDGSTLKLPEVPFPDFSPVANKGSTTSIGGAYSITATHNTKNHHSVATQNWGNSTYKQTDWNTSHPDFAVSRLDKFVVETRGATEGADISLSKQQALERYGVNYKGEKKLIAFRAGSGVVSVKKNGRITPFNEVSYKPEMLNGSFVHIDDWSGWLILTNNQFDEFNNIASQGDSGSALFVYDNQKKKWVVAGTVWGIYNYANGKNHAAYSKWNQTTIDNLKNKYSYNVDMSGAQVATIENGKLTGTGSDTTDIKNKDLIFTGGGDILLKSSFDNGAGGLVFNDKKTYRVNGDDFTFKGAGVDTRNGSTVEWNIRYDNKDNLHKIGDGTLDVRKTQNTNLKTGEGLVILGAEKTFNNIYITSGDGTVRLNAENALSGGEYNGIFFAKNGGTLDLNGYNQSFNKIAATDSGAVITNTSTKKSILSLNNTADYIYHGNINGNLDVLQHHETKKENRRLILDGGVDTTNDISLRNTQLSMQGHATEHAIYRDGAFSCSLPAPMRFLCGSDYVAGMQNTEADAVKQNGNAYKTNNAVSDLSQPDWETGTFRFGTLHLENSDFSVGRNANVIGDIQASKSNITIGDTTAYIDLHAGKNITGDGFGFRQNIVRGNSQGETLFTGGITAEDSTIVIKDKAKALFSNYVYLLNTKATIENGADVTTQSGMFSTSDISISGNLSMTGNPDKDNKFEPSIYLNDASYLLTDDSARLVAKNKASVVGDIHSTKSASIMFGHDESDLSQLSDRTSKGLALGLLGGFDVSYRGSVNAPSASATMNNTWWQLTGDSALKTLKSTNSMVYFTDSANNKKFHTLTVDELATSNSAYAMRTNLSESDKLEVKKHLSGENNILLVDFLQKPTPEKQLNIELVSAPKDTNENVFKASKQTIGFSDVTPVITTRETDDKITWSLTGYNTVANKEATRNAAALFSVDYKAFLNEVNNLNKRMGDLRDINGEAGAWARIMSGTGSASGGFSDNYTHVQVGVDKKHELDGLDLFTGFTVTHTDSSASADVFSGKTKSVGAGLYASAMVDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYSTHSWYAGAEAGYRYHVTEDAWIEPQAELVYGSVSGKQFAWKDQGMHLSMKDKDYNPLIGRTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSRMLMSVGLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF |
| Enzyme Length | 1300 |
| Uniprot Accession Number | O32591 |
| Absorption | |
| Active Site | ACT_SITE 127; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01028; ACT_SITE 156; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01028; ACT_SITE 263; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01028 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Serine protease with cytotoxic effect. Disrupts actin cytoskeleton resulting cell detachment in vitro. {ECO:0000269|PubMed:9379905}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (3); Domain (2); Signal peptide (1); Site (1) |
| Keywords | Cell outer membrane;Direct protein sequencing;Hydrolase;Membrane;Periplasm;Plasmid;Protease;Secreted;Serine protease;Signal;Transmembrane;Transmembrane beta strand;Virulence;Zymogen |
| Interact With | P0A6A8; P0A8Z3 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [Serine protease EspP]: Periplasm {ECO:0000250}.; SUBCELLULAR LOCATION: [Secreted autotransporter protein EspP]: Secreted. Cell surface.; SUBCELLULAR LOCATION: [Autotransporter protein EspP translocator]: Cell outer membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. Note=The cleaved C-terminal fragment (autotransporter domain) is localized in the outer membrane. {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | PTM: Cleaved to release the mature protein from the outer membrane. {ECO:0000250}. |
| Signal Peptide | SIGNAL 1..55; /evidence=ECO:0000269|PubMed:9379905 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 16294310; |
| Motif | |
| Gene Encoded By | Plasmid unnamed |
| Mass | 141,710 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |