| IED ID | IndEnz0002002630 |
| Enzyme Type ID | protease002630 |
| Protein Name |
D-alanyl-D-alanine carboxypeptidase DacB2 D,D-carboxypeptidase DacB2 DD-carboxypeptidase DD-peptidase EC 3.4.16.- |
| Gene Name | dacB2 Rv2911 |
| Organism | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Corynebacteriales Mycobacteriaceae Mycobacterium Mycobacterium tuberculosis complex Mycobacterium tuberculosis Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
| Enzyme Sequence | MRKLMTATAALCACAVTVSAGAAWADADVQPAGSVPIPDGPAQTWIVADLDSGQVLAGRDQNVAHPPASTIKVLLALVALDELDLNSTVVADVADTQAECNCVGVKPGRSYTARQLLDGLLLVSGNDAANTLAHMLGGQDVTVAKMNAKAATLGATSTHATTPSGLDGPGGSGASTAHDLVVIFRAAMANPVFAQITAEPSAMFPSDNGEQLIVNQDELLQRYPGAIGGKTGYTNAARKTFVGAAARGGRRLVIAMMYGLVKEGGPTYWDQAATLFDWGFALNPQASVGSL |
| Enzyme Length | 291 |
| Uniprot Accession Number | I6Y204 |
| Absorption | |
| Active Site | ACT_SITE 69; /note=Acyl-ester intermediate; /evidence=ECO:0000305|PubMed:25551456; ACT_SITE 72; /note=Proton acceptor; /evidence=ECO:0000250|UniProtKB:P0AEB2; ACT_SITE 124; /evidence=ECO:0000250|UniProtKB:P0AEB2 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by the beta-lactam antibiotic meropenem (PubMed:22906310). Inhibited by the non-specific inhibitor phenylmethylsulfonyl fluoride (PMSF) (Probable). {ECO:0000269|PubMed:22906310, ECO:0000305|PubMed:25551456}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.16.- |
| Enzyme Function | FUNCTION: Probably cleaves the terminal D-Ala-D-Ala dipeptide of the peptidoglycan stem peptide (Probable). Shows significant D,D-carboxypeptidase activity in vitro (PubMed:22906310). Acts on the synthetic penta-peptide substrate Penta-DAP (L-Ala-gamma-D-Gln-DAP-D-Ala-D-Ala). Shows also weak activity on Penta-Lys (L-Ala-gamma-Glu-L-Lys-D-Ala-D-Ala) (PubMed:22906310). The catalytic domain binds weakly to peptidoglycan in vitro (PubMed:25551456). Plays an important role in the maintenance of colony morphology and cell wall permeability and integrity (PubMed:25467937). {ECO:0000269|PubMed:22906310, ECO:0000269|PubMed:25467937, ECO:0000269|PubMed:25551456, ECO:0000305|PubMed:22906310}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis. {ECO:0000305|PubMed:25467937}. |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (11); Chain (1); Helix (10); Signal peptide (1); Turn (2) |
| Keywords | 3D-structure;Carboxypeptidase;Cell shape;Cell wall biogenesis/degradation;Hydrolase;Peptidoglycan synthesis;Periplasm;Protease;Reference proteome;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Periplasm {ECO:0000305|PubMed:25551456}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..22; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (2) |
| Cross Reference PDB | 4P0M; 4RYE; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 29,747 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |