| IED ID | IndEnz0002002579 |
| Enzyme Type ID | protease002579 |
| Protein Name |
Probable peptidoglycan endopeptidase LytE EC 3.4.-.- Cell wall-associated polypeptide CWBP33 Gamma-D-glutamate-meso-diaminopimelate muropeptidase LytE Minor autolysin LytE Phosphatase-associated protein PapQ Vegetative cell wall hydrolase LytE |
| Gene Name | lytE cwlF papQ BSU09420 |
| Organism | Bacillus subtilis (strain 168) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus subtilis group Bacillus subtilis Bacillus subtilis subsp. subtilis Bacillus subtilis (strain 168) |
| Enzyme Sequence | MKKQIITATTAVVLGSTLFAGAASAQSIKVKKGDTLWDLSRKYDTTISKIKSENHLRSDIIYVGQTLSINGKSTSSKSSSSSSSSSTYKVKSGDSLWKISKKYGMTINELKKLNGLKSDLLRVGQVLKLKGSTSSSSSSSSKVSSSSTSTYKVKSGDSLSKIASKYGTTVSKLKSLNGLKSDVIYVNQVLKVKGTSTSSSKPASSSSSSSSKTSSTSLNVSKLVSDAKALVGTPYKWGGTTTSGFDCSGFIWYVLNKQTSVGRTSTAGYWSSMKSIASPSVGDFVFFTTYKSGPSHMGIYIGNNSFIHAGSDGVQISSLNNSYWKPRYLGAKRF |
| Enzyme Length | 334 |
| Uniprot Accession Number | P54421 |
| Absorption | |
| Active Site | ACT_SITE 247; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU01284; ACT_SITE 296; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01284; ACT_SITE 308; /evidence=ECO:0000255|PROSITE-ProRule:PRU01284 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.-.- |
| Enzyme Function | FUNCTION: Cell wall hydrolase that cleaves gamma-D-glutamate-meso-diaminopimelate bonds in peptidoglycan (By similarity). Seems to play a role in cell separation during vegetative growth. {ECO:0000250, ECO:0000269|PubMed:10322020, ECO:0000269|PubMed:14594841}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Domain (4); Region (3); Sequence conflict (6); Signal peptide (1) |
| Keywords | Cell wall;Cell wall biogenesis/degradation;Direct protein sequencing;Hydrolase;Protease;Reference proteome;Repeat;Secreted;Signal;Thiol protease |
| Interact With | |
| Induction | INDUCTION: Expressed during the exponential growth phase, under the control of SigA (major) and SigH (minor). Is also positively regulated by the two-component system YycFG. {ECO:0000269|PubMed:17581128, ECO:0000269|PubMed:9573210}. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000269|PubMed:14594841}. Note=LytE is localized at cell separation sites and cell poles of vegetative cells. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..25; /evidence="ECO:0000269|PubMed:9457885, ECO:0000269|PubMed:9573210" |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 35,455 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |