| IED ID | IndEnz0002001926 |
| Enzyme Type ID | protease001926 |
| Protein Name |
Replicase polyprotein 1ab pp1ab ORF1ab polyprotein Cleaved into: Non-structural protein 1 nsp1 ; Non-structural protein 2 nsp2 ; 3C-like serine proteinase 3CLSP EC 3.4.21.- M-PRO nsp3 p27 ; Non-structural protein 4 nsp4 ; Non-structural protein 5 nsp5 ; Non-structural protein 6 nsp6 ; Non-structural protein 7 nsp7 ; Non-structural protein 8 nsp8 ; RNA-directed RNA polymerase Pol RdRp EC 2.7.7.48 nsp10 p100 ; Helicase Hel EC 3.6.4.12 EC 3.6.4.13 nsp11 p67 ; Exoribonuclease ExoN EC 3.1.13.- nsp12 ; Non-structural protein 13 nsp13 ; Uridylate-specific endoribonuclease EC 3.1.-.- NendoU nsp14 ; Putative 2'-O-methyl transferase EC 2.1.1.- nsp15 |
| Gene Name | rep 1a-1b |
| Organism | Berne virus (BEV) |
| Taxonomic Lineage | Viruses Riboviria Orthornavirae Pisuviricota Pisoniviricetes Nidovirales Tornidovirineae Tobaniviridae Torovirinae Torovirus Renitovirus Equine torovirus Berne virus (BEV) |
| Enzyme Sequence | MSTSSSILDIPSKMFRILKNNTRETEQHLSSSTLDLISKSQLLAQCFDTQEIMASLSKTVRSILESQNLEHKSTLTPYNSSQSLQLLVMNTSCTQFKWTTGSTSSVKALLEKELCRGLVPLNDITPKSNYVELSLLTPSILIGNETSTTTTLPEIPLDMEQSIISCVENTLLKEVQALSGQESCQEYFLSANYQSLIPPQVLLNLMKMSSVVDLSPLTLPNTRLWLKLSPFHGGTSVSYATQIKGYANCARREEKCLKNRLTKKQKNQEKGSFDARSVITLGGKMYRYKVVVLRCEDQSDNLSELQFEPQVEYTMDMVPHCWKELVKKRLIRAKGTWDLSCVEDLDLDHVEVRGDSLLHRSSVVHDLTSIVDDTLQEKLFSRTWLRQSLKYSGNILQRLSSLFATEGLKKITLVNSDITPVQVGDKWLNFVDFGKSTVFFVKTLNNIHLAMTRQRESCNYIHEKFGRVRWLGAKPEQGAIVKVFAWCLNKKEFKFRDNQLKQYVCRQGVIKHEPCEYLNVEVLDEFVALNNDLNCVQKIKTYLAAYFGLKKVKLTQKNFMTPLITKKQELVFQPCNCPNHQFYVAQFDKHVTLGLGRKDGILFAEQVPSYAIILAVGFGTVETQLVTHYYSEMRRVYHPLDFQSNTFVFDHQGVMLEDISPADYNDVGEEDYQLEYSGGFDQPFQNYHSDDEDQAFPDFEDERHPDEENWARPIISSGESSVVSSRPSSPLVYSSLVPVASPFGYMNGIRVFDICLADDLDFLQIHGQCPCARCKGLYFYQPIRPRGFTIFENVVEFFSFVEKCEVFEEIGPFFKMIEYSMLYNEYNIFYGLGKKIYQSDLVLPVKHLDQLWKRAQLDIDVVSEFENFKNSLQNINNVVYIAPYFNDQGEWNDIFDGYEFNLNDNQFWFQAKPVYDLVCYIYQGFFSDSRPLEKLYQKLCLDYHTSAMLHTQTHLKYCYVALLHSERAFQMSINLDSLDNEQLHFLATMGMGDASLVGPTYLSEYHSNFNWYSIMSKACHYVKLEQLVGLTYQEKRLMILSRVQEFYEQQHRGPIQLILSPLKVVNLPPITCTEGYCYQPVTRLFDTCVMPDIMKKLSRKRTSVSDVFGILADYFKRTLSYRCFKVHEFCGIERQQEFSDMTTLKLVTDWCQDTYYFYNEYATMTDVEPKVQVSSDYYLKIPSEVVEHIRQFLPHNVNVGLMNYVSSNCDFDQCKFEFCLSGKGYVLGNMFFNRCAIQYVKTNLFIVLFKSRPLLYITQESIYLSDFNVLQAQCLTGEFCLDFEPVQGKTLFGVYFTNGQRYGQQWETLPRFSLKPLNSPRKRVPTQPFEELAEVCIFKQKLKLTQLHNDCSVTPRVCSIPQTITATFQPYYCLENFYGVKAPKVIVSGHLATHYVKLTHKISKCVLVTKLAVARAFYFTPTSMGSHYHLDPMEGISFGKRATVQFEPVGLIKDVNLLVYQFGSHVSIQFFPEAPCIVADGHYPSKYSGVWLGYLPSVEECKIAQVNHRVYVPTILRTSKSAPFHIIQNGDMGRGPITVTYHYAKNFDNKSLTPMFKMFQQVFEKSKDDIFKAFNTMSLEQKKVLSHFCGEFDEAYTLQTMSDEISFESSAYPDVVACSLAYILGYEMCLTVKVNAKNEKLDIGSQCERVFVDYDVKKNEWTLSPEEGEDSDDNLDLPFEQYYEFKIGQTNVVLVQDDFKSVFEFLKSEQGVDYVVNPANSQLKHGGGIAKVISCMCGPKLQAWSNNYITKNKTVPVTKAIKSPGFQLGKKVNIIHAVGPRVSDGDVFQKLDQAWRSVFDLCEDQHTILTSMLSTGIFGCTVNDSFNTFLSNVARLDKSLVVFVVTNMVEQYNQAFAVIKMYQQYHGLPNFGNTCWFNALYQLLKSFSEKEQCVNDLLNCFDDFYDCPTSQCVEWVCEQLGVQFGQQQDAVEMLMKVFDVFKCDVRVGFDCLSRLQQVNCGFCVEVPAQAVLMFSGKDQCGHWTAARKIVDKWYTFDDNHVVQKDPVWQNVVLVLRDRGIFRSADFERKPARRRRVSHRVPRDTLSQDAITYIEDLRFSSGTCLSRYFVESVESFVSGDNVSEVSDEQTCVEVAIEESDGHVEQICQSSVDCVGMPESFQFTFSMPLQTFVQECDQKCEDDFSQEHVECDQQFEPVEQVGQGGQQDGQVDQQIKESEQVVEPSAPSGQESPQALLQQVVDEVVYQIEQVKCDQKQDQDSVQCDEIEEINSRGEQTVQQQLQPILGHDLNENEGPTLSVGAGKLVRCRSLAVTESNLSTSNTIFVWSEVLTHQYIGFKTDLMGLTYNIKFKLICYVLFLWFGVLCCTSHNTPFYMRLCIYLVLLWLSLMIWNASQINVKTGWNELYVLKLLTSIKLPNIVKFRCELVQWFVLKCLFVSFYVYDYVVKVCVSIFQMPQLRPFTWPFIKLGFVDTFLSHHILAFPEKVANQSTLPTCGDKRYYVYVPSWCRASFTSLVMRARELTSTGRSKTLDNWHYQCCSKTAKPLSCFNVREFVFDQDCKHEAYGFLSSLCVYLLFYSGFLTFWLPLFCYYYVLFMCTFKNLPVDITKPIKWTVLQQVVNDVLSLVTKPLFGRPVCPPLTTYLTSTTADEAVKVSRSLLGRFCTPLGFQQPVMNVENGVTVSNFGFFNPLMWPLFVVVLLDNRFIWFFNVLSYVMMPVFVIILFYFYLKKICGCINFKGLSKCCTKHFNQFSKPLVAAGVHGNRTNFTYQPMQEHWCDRHSWYCPKEEHYMTPDMAVYIKNYYNLACAPTADLVWCDYTKSAPTMTWSNFKYSSYKAKETVLCAPSSHADSMLMAWYALLHNVRFTVNPNVVDLPPAVNTIYVSSDSEDSVQDKSQPDVKLRPKKPKGNFKKQSVAYFSREPVDIWYYTTLVIVMGVLFMFMYSCLMVGQYVVMPRDKFFGVNPTGYSYVNAPPYLHAAPPVLQNSDGMILATQLKVPSITYSVYRLLSGHLYFTKLIVSDNECTPPFGAARLSNEFSCNGFTYVLPAHLRFFNRYVMLIHPDQLHMLPFEVEYGSHTRVCYTTGSNSVECLPTFEIISPYVFVFIVVIFTVIFLILIRLYIVMYSYFKVFTYVVFKLLFVNIIMVLFVVCLPPLVPGVVFVLALWLCDSVMFLLYLAFLSLFILPWFYVLFFLFMVGGFVFWWMMRSADVVHLTTDGLTFNGTFEQISKCVFPLNPLIVNRMLLDCQMSHSDLVEKSKLKTTEGKLANEMMKVFMTGETSYYQPSNFSFQSVFSKATSPFTLHARPPMPMFKLYVHFTGSCVGSTSTGTGFAIDDNTIVTAKHLFEYDDLKPTHVSVEIVTRSHSARSASIIWKEPDVKGWTFKGENAYIQVENLKDFYIEDFKYLPFQQIEKDFYKRMEPVTIYSVKYGSEFATQAWQTVNGHFVCYNTEGGDSGAPLVCNGRIVGVHQGLCDNFKTTLASDFEGKMMTEVKGHHVDPPVYYKPIIISAAYNKFVAGEDSSVGDGKNYHKFENEDFACMCKELESVTFGDQLRRYCYNLPQFLEPLQYFHVPSFWQPFKKQSVSNNVSWVVEHLHFIFSIYFLICDFVAYWWLDDPFSVVLPLFFIVQLLSTVFLKNVLFWTTSYLITLAVTFYIHSEVAESMFLLGFLSDRVVNRMSLIIVVAIMCLFVVVRVVVNVKRAIFVFVVSVVLIFVHICLGIVQFNSFVNVVLFDVYAVFTALLTPQPVVAIIMLLLFDTKMLMSFAFIVIVLSFRVFKDYKFVKVLHNFCNFDFVLSQVSLFRYRHRNQGNDPTHYEALWLFLKELYYGIQDAKYEVFSPQAGSYNVKFLTDMTEQDQLEAVEQVQRRLQRFNIVQDKASPRLVLYSKTIEFIKDQIQQQRAVGANPFIITTLTSNDIGLDNVEVHNPANFKPEDLQAHMWFFSKSPVFIGQVPIPTNVQTAAVLDTTYNCQDLTADEKNNVAATLQIQNAAITLSLFEKCTQFLESELGEVPTLMWQAEDVADIKHLESQIENLRKVLDGMQFGTTEYKATRKQLNICQSQLDQAKAFERKLAKFLEKVDQQQAITNETAKQLSAFKNLVKQVYESYMSSLKVKVLEANDASCLLTSTDLPRKLVLMRPITGVDGIKIVEKANGCEITAFGTTFNTGHGSNLAGLAYSTTQPLSAYPFIFNLEGIFKQQANIGYKTVECNMSSHNGSVLYKGKVVAVPSDDNPDFVVCGKGYKLDCGINVLMIPSIVRYITLNLTDHLQKQSLKPRRRLQYRQQGVRLGGVNLGEHQAFSNELISTVGYTTWVSSTVCRDNTHKHPWFVQIPVNEKDPEWFMHNTQLKDNQWVVDLKPTHWLVNADTGEQLFALSLTDEQALKAEAILQKWSPITQDVECWFKDLKGYYTVSGFQPLWPVCPVNICNVRLDPVFKPQSIVYADDPTHFLSLPVVNKNFLAAFYDLQEGFPGKKQVAPHISLTMLKLSDEDIEKVEDILDEMVLPNSWVTITNPHMMGKHYVCDVEGLDSLHDEVVSVLREHGIACDQKRLWKPHLTIGELNDVSFDKFKDFAISCKLEDCDFVKLGAPKANARYEFITTLPLGDFKLLRGAWSACRHLCFQNGAYQSSRSKHYIDLATEYNAGIVKVNKSNTHSVEYQSKRFMIKRVKDQSEFALAKTAFLPSIIPHHMEKQNGEWFLIRGPTSQWSLGDLVYAIWLGDQDYLSECGFVFNPSRDEFLDDANQRSFLANLLEPAILNFSHIYWQVKMCKVPYKLTLDNVDLNGQLYDFGDYPCPNSVDNQSALFVLAEVWSMTRRPFPVAFARLLANEMEIPTDYQMFFQNILLSGSYLDKALCLNNVRPFLSDPANLTTTPFFSQHNGVWTHFYNPIYGLVECNLDEFAELPEVLQQLVTVQGPITNNMTPAISVGEGVYAANVPSASATKQKIPFYDVGLCQELTDAGVDCGEAFKYFYYLSNPAGALADVCYYDYQGTGFYSPKLLAGVYDFMKRVTECYRINERFTYEQAKPRKSSMGINITGYQQDAVYRALGPENIARLFEYAQKAPLPFCTKIITKFALSAKARARTVSSCSFIASTIFRFAHKPVTSKMVEVAQNSGGFCLIGVSKYGLKFSKFLKDKYGAIEGFDVFGSDYTKCDRTFPLSFRALTAALLYELGEWDEKSWLYLNEVNSYMLDTMLCDGMLLNKPGGTSSGDATTAHSNTFYNYMVHYVVAFKTILSDLSEGNKVMRIAAHNAYTTGDYQVFNTLLEDQFQTNYFLNFLSDDSFIFSKPEALKIFTCENFSNKLQTILHTKVDQTKSWSTKGHIEEFCSAHIIKTDGEYHFLPSRGRLLASLLILDKLSDVDIYYMRFVAILCESAVYSRYQPEFFNGLFQVFLDKVQQFRKDYCCDPCPPQLLEREFYENLVFTSNSEVGIVDCYLENFKLQCEFKQQANFDKVCFCCPNPAVSVCEECYVPLPLCAYCYYVHVVISNHSKVEDKFKCFCGQDNIRELYIVLNNSICMYQCKNCVESDRLRISLLSDVDQIVRLPGFKSNSASIAKNGVAQLLTSVDNVDVSLDWNYQESVQQNVARIVYHSANMTQMSIEVVYVSFTLVRNDGSSAILDIPNFKCPDTSYCLFYKPGKSGVLKFTGKGTLTSCYDNKNLTWFKVTCPDFNQPWRLATCFVIQQHDVVYPPIKATQYENVTFVMGPPGTGKTTFVYDTYLSKASSSNRFVYCAPTHRLVGDMDEKVDGAVVVSAYNDRTYRNPVWNKDDSYGVLLCTHNTLPFIKSAVLIADEVSLIPPHVMIKILSMGFKKVVLLGDPFQLSPVYKNHKVHFKYDTFYLLQLATQKRYLTACYRCPPQILSAFSKPYCDVGVDLVSFNNKPGKFDIIVSKQLANIQDFSVLSVLSKEYPGYVILVNYRAAVDYAMQNGLGDVTTIDSSQGTTAANHLLVLFGASNFSKTVNRVIVGCSRSTTHLVVVCCPELFKHFQPILNWPEPKYRYFGMEKQSDFNIIPEVSSLVFCDIEFWHYKADPNSKTRTVYPGQIAVVTSQTLQLYLGVFDDTGYKSALRGLPKDVYVPPNWVWMRKHYPSYEQHAYNMQRLFKFIIDTTFGQPWFILYSCSNDLKSLKFYVEFDTCYFCSCGEMAICLMRDGNYKCRNCYGGMLISKLVNCKYLDVQKERVKLQDAHDAICQQFHGDSHEALCDAVMTKCLYLASYEAAFKDTIHVKYKDLCLEIQYKITSSFVRYDSVHKRYLYRDHGAMYYFRTPRSPMQNVYKYEVGSHAEYSINICTSYEGCQSFGKTCTKCIHIHCIVEQFMADERFKEFILVSVVKSDYVEQALSPAAKALMLTVTKVEDKSFYISNGVRYDLYDYDLSKSVMRVVNSNVKPLPLYSVIVGLGINCTVGCVLPNVPMKLKDELLITDVPLSTLRLDLQTWYYISWPTLSNKNSRWKLAGAQVYDCSVHIYIEATGEQPLYYLQQGKGESLFELPDTLFSTGRLYNLDHDAAQNFNVKQLAIETMPNNHHVFSGDFTEVGTDIGGVHHVVALNGYKGSIIPNYVKPIATGLINVGRAVKRTTLVDVCANQLYEKVKQQLEGVKVSKVIFVNIDFQDVQFMVFANGEDDIQTFYPQKDFVRSYYEWPNILPQIESHYDLKNYGQNPTFMPQPVNFAKYTQICTFIQDHVKVARNALVWHLGAAGVDGCSPGDIVLSSFFKECLVYSWDIKDYSTLLDKHSYDCNFRPNLIVSDIYNVSSNVSEVLDDCVHRLALGGTIVFKTTESSRPDIQLSQFTKYFSAVQFFTAGVNTSSSEVFVVLKYKLYSEPIGEELCSPNILSRIAAYRNKLCIVPNFKVFSTSFSYKYSGVKFVQKCFYVSVPRQFCASGLIQEVPMLCQMEH |
| Enzyme Length | 6857 |
| Uniprot Accession Number | P0C6V7 |
| Absorption | |
| Active Site | ACT_SITE 3304; /note=Charge relay system; for 3C-like serine proteinase activity; /evidence=ECO:0000255; ACT_SITE 3347; /note=Charge relay system; for 3C-like serine proteinase activity; /evidence=ECO:0000255; ACT_SITE 3416; /note=Charge relay system; for 3C-like serine proteinase activity; /evidence=ECO:0000255; ACT_SITE 5984; /evidence=ECO:0000255|PROSITE-ProRule:PRU01298; ACT_SITE 5986; /evidence=ECO:0000255|PROSITE-ProRule:PRU01298; ACT_SITE 6085; /evidence=ECO:0000255|PROSITE-ProRule:PRU01298; ACT_SITE 6161; /evidence=ECO:0000255|PROSITE-ProRule:PRU01298; ACT_SITE 6166; /evidence=ECO:0000255|PROSITE-ProRule:PRU01298; ACT_SITE 6487; /evidence=ECO:0000255|PROSITE-ProRule:PRU01303; ACT_SITE 6504; /evidence=ECO:0000255|PROSITE-ProRule:PRU01303; ACT_SITE 6536; /evidence=ECO:0000255|PROSITE-ProRule:PRU01303; ACT_SITE 6633; /evidence=ECO:0000255|PROSITE-ProRule:PRU01300; ACT_SITE 6709; /evidence=ECO:0000255|PROSITE-ProRule:PRU01300; ACT_SITE 6737; /evidence=ECO:0000255|PROSITE-ProRule:PRU01300; ACT_SITE 6771; /evidence=ECO:0000255|PROSITE-ProRule:PRU01300 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539}; CATALYTIC ACTIVITY: Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12; CATALYTIC ACTIVITY: Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13; |
| DNA Binding | |
| EC Number | 3.4.21.-; 2.7.7.48; 3.6.4.12; 3.6.4.13; 3.1.13.-; 3.1.-.-; 2.1.1.- |
| Enzyme Function | FUNCTION: The 3C-like serine proteinase is responsible for the majority of cleavages.; FUNCTION: The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. {ECO:0000250}.; FUNCTION: The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction. {ECO:0000250}.; FUNCTION: NendoU is a Mn(2+)-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond. {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (15); Chain (15); Domain (9); Erroneous gene model prediction (2); Metal binding (16); Region (4); Sequence conflict (1); Site (13); Transmembrane (17) |
| Keywords | ATP-binding;Endonuclease;Exonuclease;Helicase;Host membrane;Hydrolase;Membrane;Metal-binding;Methyltransferase;Nuclease;Nucleotide-binding;Nucleotidyltransferase;Protease;RNA-directed RNA polymerase;Repeat;Ribosomal frameshifting;Thiol protease;Transferase;Transmembrane;Transmembrane helix;Viral RNA replication;Zinc;Zinc-finger |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [Non-structural protein 1]: Host membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.; SUBCELLULAR LOCATION: [Non-structural protein 2]: Host membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.; SUBCELLULAR LOCATION: [Non-structural protein 4]: Host membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | PTM: Specific enzymatic cleavages in vivo by its own protease yield mature proteins. 3CL-PRO is autocatalytically processed (By similarity). {ECO:0000250}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 784,289 |
| Kinetics | |
| Metal Binding | METAL 5417; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00986; METAL 5420; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00986; METAL 5428; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00986; METAL 5431; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00986; METAL 5438; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00986; METAL 5441; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00986; METAL 5445; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00986; METAL 5451; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00986; METAL 5460; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00986; METAL 5462; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00986; METAL 5479; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00986; METAL 5483; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00986; METAL 6149; /note=Zinc 4; /evidence=ECO:0000255|PROSITE-ProRule:PRU01298; METAL 6153; /note=Zinc 4; /evidence=ECO:0000255|PROSITE-ProRule:PRU01298; METAL 6157; /note=Zinc 4; /evidence=ECO:0000255|PROSITE-ProRule:PRU01298; METAL 6172; /note=Zinc 4; /evidence=ECO:0000255|PROSITE-ProRule:PRU01298 |
| Rhea ID | RHEA:21248; RHEA:13065 |
| Cross Reference Brenda |